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By Herbert S. Rosenkranz, Elena C. McCoy, Monica Anders, William T. Speck, David Bickers (auth.), Michael D. Waters, Stephen Nesnow, Joellen L. Huisingh, Shahbeg S. Sandhu, Larry Claxton (eds.)
vi Williamsburg, Virginia, February 21-23, 1978. This symposium used to be backed by means of the U. S. Environmental safety business enterprise, workplace of strength Minerals and undefined, Washington, DC, and workplace of well-being and Ecological results, healthiness results Re seek Laboratory, Biochemistry department, study Triangle Park, NC. The symposium consisted of 24 formal displays that magnify the 3 significant issues mentioned in the course of the symposium: an summary of momentary bioassay platforms; present technique concerning the gathering and chemical research of environmental samples; and present examine in volving using momentary bioassays within the fractionation and research of complicated environmental combos. the aim of this symposium used to be to offer the state of the art tech niques in bioassay and chemical research as utilized to com plex combos and to foster persisted development of this significant quarter. complicated combos mentioned comprise ambient air and water, waste water, consuming water, shale oil, syn thetic fuels, car exhaust, diesel particulate, coal fly ash, cigarette smoke condensates, and nutrients items. it's our wish that this quantity will function a refer ence to catalyze and inspire additional learn during this box. Michael D. Waters, Ph. D. Stephen Nesnow, Ph. D. vii Acknowledgment we wish to thank Gerald Rausa, workplace of power Minerals and undefined, for his suggestion, encouragement, and help of this software. we might additionally wish to convey our appreciation to Wendy A. Martin, Peter A. Murphy, and David F. Wright of Kappa structures, Inc.
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Vi Williamsburg, Virginia, February 21-23, 1978. This symposium used to be subsidized by way of the U. S. Environmental security enterprise, place of work of strength Minerals and undefined, Washington, DC, and workplace of healthiness and Ecological results, future health results Re seek Laboratory, Biochemistry department, learn Triangle Park, NC.
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Extra info for Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures
Some of these, however, just as with the saIffionella system, can be overcome by incorporating a cell-free activation system into the assay procedure (83,87). Although this assay works quite well for readily diffusible substances (Table 9, group I), it does not yield interpretable results with poorly diffusible molecules (Table 9, group II). 13 11 moles 250 Ilg 1 Ilg 8 62 30 26 18 62 30 26 18 44 0 45 0 2 0 0 12 60 20 85 13 46 16 0 21 15 12 30 32 22 37 0 0 22 8 16 23 N-methyl-N'-nitro-N-Nitrosoguanidine plates for the determination of the number of viable cells.
The methodologies for determining cytotoxicity and mutagenic activity with the tissue homogenate-mediated system are similar to those described above for the cell-mediated assay. Umeda and Saito (34) developed a system for the microsome-mediated mutagenesis of FM3A cells, a C3H mouse mammary carcinoma cell line, using the carcinogenic nitrosamine DMN. Microsomes were prepared from mouse livers, and mutations were detected by the conversion from AZ sensitivity to AZ resistance. After treatment of the cells in suspension, they were seeded into agar containing AZ.
Hutton J, Hackney C: Metabolism of cigarette smoke condensates by human and rat homogenates to form mutagens detectable by Salmonella typhimurium TA1538. Cancer Res 35:2461-2468, 1975 49. Kier D, Yamasaki E, Ames BH: Detection of mutagenic activity in cigarette smoke condensates. Proc Natl Acad Sci US 71:4159-4163, 1974 50. Kuhr RJ: The formation and importance of carbamate insecticide metabolites as terminal residues. In: Pesticide Terminal Residues. London, Butterworth and Co, pp 199-220, 1971 51.
Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures by Herbert S. Rosenkranz, Elena C. McCoy, Monica Anders, William T. Speck, David Bickers (auth.), Michael D. Waters, Stephen Nesnow, Joellen L. Huisingh, Shahbeg S. Sandhu, Larry Claxton (eds.)